Paderborn's stay in the US capital will run from 25 September to 1 October, with additional marketing activities also planned.
"We're really looking forward to the trip to the U.S. and the opportunity to represent our sports club, the Bundesliga brand, and German soccer. The match against D.C. United is a great opportunity," said Paderborn sporting director Sebastian Lange.
"The days in Washington, D.C., will also be a very special experience for the team and the entire delegation. Among other things, we'll visit local institutions and immerse ourselves in the daily lives of the people there."
Watch: Paderborn played Minnesota United on their last US tour